CloneWeaver® Workflow Purchasing Tool

Powering Your Cloning Workflow

Product selection has never been easier

Molecular Cloning is the process of producing recombinant DNA and transforming into host organisms to replicate and make more copies. Every cloning project is unique. Read More

Once you have designed the cloning scheme, gathering all of the required reagents to get you from construct to expression and analysis is not trivial. CloneWeaver helps you build a customized cloning "kit" with all of the items your cloning scheme requires. Purchase your selection instantly, save it or email it to a purchasing agent. Soon you'll have everything you require to create the construct you need.

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Restriction Enzymes

Name Incubation Conditions Description Part Number
4-CORE Buffer Pack Open/Close Add
Majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers

The 4-CORE Buffer Pack contains convenient aliquots of Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers.

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AgeI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix

A/CCGG T T GGCC\A

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AluI Open/Close Add
Capable of digesting DNA in 15 minutes or less

AG/CT TC\GA

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BamHI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications

G/GATC C C CTAG\G

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BclI Open/Close Add
Genome qualified to ensure optimal performance in genomic analysis applications

T/GATC A A CTAG\T

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BglI Open/Close Add
Genome qualified to ensure optimal performance in genomic analysis applications

GCCN NNN/NGGC CGGN\NNN NCCG

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ClaI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

AT/CG AT TA GC\TA

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DpnI Open/Close Add
Capable of digesting DNA in 15 minutes or less

G(me)A/TC CT\(me)AG

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EcoRI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Available at high concentration, containing 25,000 units of EcoRI at a concentration of 40–80u/μl

G/AATT C C TTAA\G

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EcoRV Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

GAT/ATC CTA\TAG

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HindIII Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

A/AGCT T T TCGA\A

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HinfI Open/Close Add
Restriction endonuclease from Haemophilus influenzae Rf

G/ANT C C TNA\G

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KpnI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

G GTAC/C C\CATG G

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MluI Open/Close Add
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

A/CGCG T T GCGC\A

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MULTI-CORE Buffer Pack Open/Close Add
Enables digestion of DNA with more than one enzyme in a single reaction
Only modest adjustments in the amount of enzyme used will ensure complete multiple digestions

The MULTI-CORE Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE Buffer.

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NcoI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

C/CATG G G GTAC\C

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NdeI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix

CA/TA TG GT AT\AC

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NheI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Genome qualified to ensure optimal performance in genomic analysis applications

G/CTAG C C GATC\G

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NotI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

GC/GGCC GC CG CCGG\CG

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PstI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, provides a higher level of quality control for enzymes used in cloning applications

C TGCA/G G\ACGT C

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PvuI Open/Close Add
Active and capable of digestion directly in GoTaq® Green Master Mix

CG AT/CG GC\TA GC

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RsaI Open/Close Add
Capable of digesting DNA in 15 minutes or less

GT/AC CA\TG

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SacI Open/Close Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

G AGCT/C C\TCGA G

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SalI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

G/TCGA C C AGCT\G

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SgfI Open/Close Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

GCG AT/CGC CGC\TA GCG

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SmaI Open/Close Add
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

CCC/GGG GGG\CCC

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SpeI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

A/CTAG T T GATC\A

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TaqI Open/Close Add
Restriction endonuclease from Thermus aquaticus YTI

T/CG A A GC\T

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XbaI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

T/CTAG A A GATC\T

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XhoI Open/Close Add
Capable of digesting DNA in 15 minutes or less
Active and capable of digestion directly in GoTaq® Green Master Mix
Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Genome qualified to ensure optimal performance in genomic analysis applications

C/TCGA G G AGCT\C

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