SIRPα/CD47 Blockade Bioassay, Fc-Dependent Technical Manual
Instructions for Use of Product(s)
JA4801, JA4805, JA4901, JA4905
Literature # TM703
The SIRPα/CD47 Blockade Bioassay, Fc-Dependent is a bioluminescent reporter cell-based assay that is easy-to-use, quantitative and demonstrates the functional response of SIRPα/CD47 inhibitors bearing a functional Fc domain. It can be used to measure the potency and
stability of antibodies and other Fc-functional biologics that block SIRPα/CD47 interaction. The assay consists of two genetically engineered cell lines:
• SIRPα Effector Cells: A monocytic cell-line with a stably integrated NanoLuc® luciferase reporter driven by FcγR and SIRPα/CD47 pathway-dependent response elements.
• CD47 CHO-K1 Target Cells: CHO-K1 cells engineered to express human CD47.
The SIRPα Effector Cells and CD47 CHO-K1 Target Cells are provided in thaw-and-use format, as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation. When the two cell types are cocultured, SIRPα/CD47 interaction prevents FcγR signaling and promoter-driven luminescence. Addition of Fc-functional anti-CD47 blocking antibody disrupts SIRPα/CD47 interaction and activates FcγR, resulting in promoter-driven luminescence.
This Technical Manual is relevant for Cat.# JA4801 and JA4805; JA4901 and JA4905 (Korea).
Summary of Changes
The following changes were made to the 6/25 revision of this document:
1. Corrected information in Section 1, Description.
2. Updated a third party trademark.
Revised 6/25.