Bone DNA Extraction Kit Technical Manual
Instructions for Use of Product(s)
DC6051
Literature # TM691
Bone and teeth are traditionally considered difficult DNA extraction sample types due to their unique composition. Typical preprocessing lysis reagents used for casework samples are not effective in efficiently extracting DNA from the calcium matrix. This Bone DNA Extraction protocol is based on a Demineralization Buffer protocol developed by the Armed Forces DNA Identification Lab, and has been used in combination with many purification protocols, including organic extraction.
The Bone DNA Extraction Kit contains reagents needed for preprocessing pulverized bone samples. The preprocessing step can be followed up with automated (Maxwell® Instrument) or manual methods of DNA purification using DNA IQ™ chemistry. Protocols for both methods are included in this technical manual.
Summary of Changes
The following changes were made to the 8/22 revision of this document:
1. Added Note in Section 4.
2. Made minor text edits in Sections 4.A, 4.B and 9.B.
3. Added Related Products, Section 10.
4. Updated trademark information and document font.
Revised 8/22.