pGL4 Luciferase Reporter Vectors Technical Manual

Instructions for Use of Product(s)
E1310, E1320, E1340, E1350, E1360, E6651, E6661, E6671, E6681, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771, E6881, E6911, E6921, E6931, E8411, E8421, E8431, E8441, E8451, E8461, E8471, E8481, E8491

Literature # TM259

The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available, including those with the synthetic luc2 (Photinus pyralis) and hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells. Furthermore, both the reporter genes and the vector backbone, including the bla (β-lactamase or Amp_r) gene, have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription.

Summary of Changes
The following changes were made to the 7/25 revision of this document:
1. Removed discontinued products Cat.# E7501, E7511 and E7521.
2. Added back Cat.# E6881 to Section 2.
3. Edited Figures 5, 6 and 7 and Tables 2 and 3 to remove information about discontinued hRluc Rapid Response™ pGL4 Reporter Vectors.
4. Noted which vectors were discontinued in Table 5.
5. Updated patent/license statements and related products.
6. Updated the cover page and document font.
7. Made minor text edits.

Revised 7/25.

Complete Protocol
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