TnT® T7 Quick for PCR DNA
Coupled Transcription/Translation System for Optimum Protein Expression from PCR Templates
- Express protein directly from PCR, no cleanup needed
- Up to 5 times more protein than standard translation reactions with linear templates
- Includes Recombinant RNasin® Ribonuclease Inhibitor
Catalog Number:
Size
Catalog Number: L5540
Protein from PCR Templates, No Clean Up Required
The TnT® T7 Quick for PCR DNA System is a simple, single-tube coupled transcription/translation system designed for optimum protein expression directly from PCR templates, no cleanup needed. For most PCR templates, the reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction or purified by numerous commercially available kits and traditional methods.
Reagents including Recombinant RNasin® Ribonuclease Inhibitor are included in the Quick Master Mix.
For more information, see the Protein Expression chapter of the Protocols & Applications Guide.
Reference
- Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Methionine |
L118A | 1 × 50μl | 1mM | |
|
TnT® T7 PCR Quick Master Mix |
L554A | 8 × 200μl | ||
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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