Wizard® Plus SV Minipreps DNA Purification Systems
1–20μg High-Quality Plasmid DNA from 1–10ml Cultures
- Process up to 20 minipreps in 45 minutes
- Choice of spin or vacuum purification formats
- No phenol extractions or precipitations required
Catalog Number:
Size
Additional options
Catalog Number: A1330
Catalog Number: A1460
Catalog Number: A1465
Catalog Number: A1340
Catalog Number: A1470
Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® Plus SV Minipreps Start-Up Kits are available. Purchase a Wizard® Plus SV Minipreps DNA Purification System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.
The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector and can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129A | 1 × 50/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
|
Column Wash Solution (CWA) |
A131B | 1 × 20ml | View Product |
|
Alkaline Protease Solution |
A144A | 1 × 550μl | View Product |
|
Neutralization Solution (NSB) |
A148B | 1 × 30ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711P | 1 × 20ml | View Product |
|
Cell Lysis Solution (CLA) |
A712P | 1 × 20ml | View Product |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 1 × 250/pk | |
|
Collection Tubes |
A130B | 1 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 1 × 100ml | View Product |
|
Alkaline Protease Solution |
A144B | 1 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 1 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 1 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 1 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 4 × 250/pk | |
|
Collection Tubes |
A130B | 4 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 4 × 100ml | View Product |
|
Alkaline Protease Solution |
A144B | 4 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 4 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 4 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 4 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 2 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129A | 1 × 50/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
|
Column Wash Solution (CWA) |
A131B | 1 × 20ml | View Product |
|
Vacuum Adapters |
A1331 | 1 × 20 each | |
|
Alkaline Protease Solution |
A144A | 1 × 550μl | View Product |
|
Neutralization Solution (NSB) |
A148B | 1 × 30ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711P | 1 × 20ml | View Product |
|
Cell Lysis Solution (CLA) |
A712P | 1 × 20ml | View Product |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 1 × 250/pk | |
|
Collection Tubes |
A130B | 1 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 1 × 100ml | View Product |
|
Vacuum Adapters |
A1331 | 1 × 20 each | |
|
Alkaline Protease Solution |
A144B | 1 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 1 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 1 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 1 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
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