MyGlo® Reagent Reader - Portable 96-Well Luminescence Plate Reader
Simplify Setup and Analysis of Cell Health Assays with a Personal 96-Well Device Powered by the ProNect® Data Platform
- Compact 96-well plate reader with simple setup and rapid luminescent assay results. See how it works. What scientists are saying.
- More than a plate reader - works with a wide range of luminescent assays, with full value unlocked through Promega ProNect® apps. Assay sensitivity data. Discover the Apps.
- Guided app workflow for experiment setup, plate reading, and cloud-based analysis. See example results.
- Free 10ml sample of CellTiter-Glo® 2.0 Reagent included with your purchase. Discover MyGlo® Special Offers.
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Streamlined Luminescence Results, Start to Finish
MyGlo® is more than a compact plate reader—it’s part of Promega’s ProNect® Data Platform , delivering guided flexible workflows built by scientists for scientists.
- From read to results in less than 30 seconds for a full plate read (1s integration time).
- ProNect® apps guide you through plate setup, reading, and analysis.
- Automated calculations generate clear, accurate results in minutes.
- Cloud-based analysis provides access to interactive graphs and detailed data, wherever you are.

It's As Simple As It Looks,
Maybe Simpler

Automated In-App Analysis: From Assay to Insight in Minutes
Turn raw luminescence data into clear results—no manual calculations, no extra software.
ProNect® apps work with MyGlo® to guide you from plate read to analysis, delivering rapid, ready-to-use insights. For Promega assays with dedicated apps, ProNect® automatically delivers:
- Analysis types: dose response (with EC50/IC50 values and 4PL/5PL curve fits), single concentration, and linearity analysis.
- Assay-specific visualizations, including:
- CellTiter-Glo®: cell viability and % inhibition
- Caspase-Glo® 3/7: caspase activity
- LDH-Glo™: % cytotoxicity and LDH release
- Exportable Data: Raw and processed data accessible in-app or exportable to Excel for downstream use.
What You Need to Know About MyGlo®
- Software included via ProNect®: Guided workflows for plate setup, reading, analysis, and cloud-based results.
- Incubator ready: Compatible with use in incubators. (See Data)
- No calibration required: Factory calibrated, advanced sensor technology ensures accuracy, and each device includes a calibration certificate.
Special Offers
Promega is excited to offer a range of special promotions to help you bring the MyGlo® plate reader and our ProNect® apps to your lab.
Starting a new lab?
We’re here to help. Get 20% off MyGlo® through Promega’s New Lab Startup Program and simplify luminescence from day one.
Preparing the next generation of scientists?
Promega’s Training Support Program offers special MyGlo® bundles to make classroom learning more engaging and accessible.
Use it. Love it. Keep it.
Our Try Before You Buy program puts MyGlo® in your lab for 60 days - free.
MyGlo® FAQs
- Single-mode design that streamlines operation for specific assays.
- Remote control capabilities let you manage experiments from anywhere.
- Meaningful data insights are surfaced through guided setup, plate reading, and data analysis all managed through ProNect® apps.
- Always available—MyGlo is ready when you’re ready, eliminating the wait and planning required for shared instruments.
MyGlo® gives you the flexibility of two different workflows depending on the luminescent assay you are using and on the requirements of your experiment.
Workflow 1: Plate Read + Analysis
Provides assay-specific results and end-to-end data analysis in less time than incubating CellTiter-Glo® samples. Available for assays with dedicated ProNect® apps (e.g., CellTiter-Glo®).
Steps:
- Quick read in <30 seconds.
- Create a plate map (~5 minutes).
- Analyze and visualize data (~10 seconds).
Workflow 2: Quick Read + Export
Works with any luminescent assay, including customized assays without a dedicated ProNect® app.
Steps:
- Read a full 96-well plate in <30 seconds.
- See results instantly as a raw RLU heatmap.
- Easily export data as Excel file (GraphPad Prism–ready).
3D Spheroid Compatibility
- MyGlo® is compatible with 3D luminescent assays.
- For best results, transfer spheroids from a seeding plate to a white 96-well plate before measurement.
MyGlo® is designed to leverage the power and convenience of the cloud. It requires an internet connection via your lab’s Wi-Fi to operate using the ProNect® apps.
- Currently, there is no offline version of MyGlo®.
- Coming soon: Glo-Fi™ network accessory, which will expand connectivity options to Wi-Fi 5Ghz, wired Ethernet, and global cellular.
Instrument Specifications
Requirements for Operation | |
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Wi-Fi Connection |
MyGlo® requires connection to the internet via a Wi-Fi network to operate. Please see MyGlo® Setup Instructions for specific Wi-Fi connectivity requirements. |
Control and User Interface |
MyGlo® is controlled using ProNect® apps in the ProNect® Data Platform and requires a free ProNect® account. Create a ProNect® account. The ProNect® Data Platform works with common desktop internet browsers, including Microsoft Edge®, Apple Safari®, and Google Chrome®. Mobile and tablet browser experiences not supported at this time. |
Setup and Management |
The Connect2ProNect is a desktop application for setup and management of MyGlo® and runs on Microsoft® Windows 10+. MacOS® and Virtual Machines not supported. |
Performance | |
Linear Dynamic Range | 6 logs |
Cross Talk | Average <0.02% |
Sensitivity | Assay Sensitivity Data |
System | |
Dimensions |
98mm × 140mm × 42mm (W × L × H) |
Weight | 570g (1.25lb) |
Regulatory Compliance | For Research Use Only. Not for use in diagnostic procedures. |
Power Supply |
5V |
Power Input | 2.5W |
Warranty | 12 months; see Operating Manual for details. |
Environmental | |
Shipping Temperature | +4°C to +50°C |
Operating Temperature | +15°C to +40°C |
Operating Relative Humidity | ≤80% noncondensing |
Operating Altitude | <2,000m |
Specifications
Catalog Number:
What's in the box?
Item | Part # | Size | Available Separately |
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MyGlo™ Reagent Reader
|
MG1000 | 1 × 1 each | |
CellTiter-Glo® 2.0 Reagent |
G9241 | 1 × 10ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.SDS
Search for SDSCertificate of Analysis
Operating Manual
Complete Protocol
Resources
Articles
Software and Firmware
Other Resources
Service and Support
MyGlo® Reference Materials |
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Helpful guides and instructions to get you started using MyGlo®. MyGlo® Setup Instructions - PDF Setting Up Your MyGlo® - Video |
One Call Supports It All |
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We supply both the reagents and the instrument, so one call to Promega answers any questions you may have. |
If you need additional coverage during or after the MyGlo® Reagent Reader standard warranty period is over, a MyGlo® Reagent Reader Service Agreement is available. The MyGlo® Reagent Reader Service Agreement covers all parts, labor and shipping to and from our authorized service locations. This Agreement does not include repair or replacement necessitated by accident, neglect, misuse, unauthorized repair or modification of the instrument.
If a problem arises with the instrument, please contact Promega Technical Service for support. E-mail: techserv@promega.com. If further action is required, you will be given repair options and assigned a return authorization number. Promega is not responsible for instrumentation returned without an authorization number.
To obtain service during the covered period, please take the following steps:
- Contact Promega Technical Services.
- Carry out tests as suggested by your Technical Services contact.
If it is determined that further action is required, you will be given options and assigned a return authorization number. Promega is not responsible for instrumentation returned without an authorization number.
Before returning the instrument, you will be responsible for cleaning it. If the instrument has been exposed to any chemical hazards, consult the Technical Manual for cleaning instructions.
*May not be available in all countries. Contact your local Promega representative for more information.
Your MyGlo® Reagent Reader instrument comes with a 12-month* warranty. Warranty
coverage includes all parts, labor, and shipping to and from our authorized service locations.
The warranty does not include repair or replacement necessitated by accident, neglect, misuse,
unauthorized repair or modification of the instrument.
If a problem arises with the instrument, contact Promega Technical Services for support. Promega Technical Services can be contacted directly from the ProNect® dashboard or via
email: techserv@promega.com. If
further action is required, you will be given repair options and assigned a return authorization
number. Promega is not responsible for instrumentation returned without an authorization
number.
The detection system is not fully sealed. Excessive liquid can enter the unit and damage internal electronic components. Ensure proper care when handling liquids near the system to prevent potential damage.
To obtain service during the warranty period, please take the following steps:
- Contact Promega Technical Services.
- Carry out troubleshooting procedures as suggested by your Technical Services Scientist.
- If further action is required, you will be provided options and assigned a return authorization number. Promega is not responsible for instrumentation returned without an authorization number.
- Before returning the instrument, you will be responsible for cleaning it. If the instrument has been exposed to any chemical, biological or radioactive hazards, contact Promega Technical Services for decontamination instructions before shipping.
*May vary based on location. Contact your local Promega representative for more information.
Related Products
Frequently Used With
CellTiter-Glo® 2.0 Cell Viability Assay
Updated CellTiter-Glo® Cell Viability Assay with improved reagent stability. Quantifies cell proliferation based on ATP detection.
G9241, G9242, G9243, MG1010
CellTiter-Glo® One Solution Assay
Gold standard method for determining cell viability based on quantitation of ATP.
G8461, G8462
CellTiter-Glo® Luminescent Cell Viability Assay
Determine the number of viable cells in culture based on quantitation of ATP present.
G7570, G7571, G7572, G7573, MG1010
CellTiter-Glo® 3D Cell Viability Assay
A homogeneous method optimized to assess viability in 3D cell culture.
G9681, G9682, G9683
Caspase-Glo® 3/7 Assay System
An easy-to-use, plate-based luminescent assay for detecting caspase-3/7 activity.
G8090, G8091, G8093, G8092, MG1010
Caspase-Glo® 3/7 3D Assay
A simple luminescent assay that measures caspase-3 and -7 activities in 3D cultures.
G8981, G8982, G8983
LDH-Glo™ Cytotoxicity Assay
Bioluminescence-based LDH assay for sensitive detection of cytotoxicity in samples low in cell number, including 3D microtissues.
J2380, J2381