The Pgp-Glo™ Assay Systems are luminescent P-glycoprotein (Pgp) ATPase assays. The Pgp-Glo™ Assay detects the effects of compounds on recombinant human Pgp in a cell membrane fraction. The assay couples the ATPase activity of Pgp to the ATP dependence of the light-generating reaction of firefly luciferase. The rate of ATP consumption by Pgp is inversely correlated with the light output of the luciferase reaction. The Pgp-Glo™ Assay is easily amenable to high-throughput screening and can complement cell-based Pgp studies.
Promega Notes 96, 11–14.
Dongping Ma and James J. Cali