Promega Corporation

Primer Extension System—AMV Reverse Transcriptase

Primer Extension System—AMV Reverse Transcriptase can be used to quantitate specific mRNA transcripts and map the start sites of transcription. An end-labeled oligonucleotide is hybridized to RNA and is used as a primer by reverse transcriptase in the presence of deoxynucleotides. The RNA is thus reverse transcribed into cDNA and is analyzed on a denaturing polyacrylamide gel. The length of the cDNA reflects the number of bases between the labeled nucleotide of the primer and the 5´-end of the RNA; the quantity of cDNA product is related to the amount of targeted RNA.

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Primer Extension System-AMV Reverse Transcriptase

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Primer Extension System-AMV Reverse Transcriptase

 Components

  • 1.2kb Kanamycin Positive Control RNA

    C154A1 x 100ng
  • Loading Dye

    E343B1 x 1ml
  • Primer Extension 2X Buffer

    E349B1 x 500μl
  • Primer Extension Sodium Pyrophosphate

    E350B1 x 75μl
  • ΦX174 DNA/HinfI Dephosphorylated Markers

    E351A1 x 500ng
  • Primer Extension Control Primer

    E355A1 x 50pmol
  • AMV Reverse Transcriptase

    E357B1 x 50u
  • T4 Polynucleotide Kinase

    M410A1 x 100u
  • T4 Polynucleotide Kinase 10X Buffer

    Q561A1 x 100μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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40 reactions
- E3030 Please Enquire


Storage Conditions

All components must be stored at –20°C, except for the control RNA, which must be stored at –70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

E3030 For Research Use Only. Not for Use in Diagnostic Procedures.

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