Promega Corporation

GloResponse™ Luciferase Reporter Cell Lines

The GloResponse™ Luciferase Reporter Cell Lines contain optimized, state-of-the-art luciferase reporter technology integrated into a cell line. This allows the rapid development of a reporter assay based on the pathway of interest regulating the luciferase gene. Assays configured using the GloResponse™ Cell Lines are amenable for high-throughput screening. These assays typically have greater response dynamics (fold of induction) than other assay formats and good quality as indicated by the high Z´ values. GloResponse™ Cell Lines were developed to study a variety of signaling pathways. Activators of these pathways ...

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GloResponse™ CRE-luc2P HEK293 Cell Line

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GloResponse™ CRE-luc2P HEK293 Cell Line

 Components

  • GloResponse™ CRE-luc2P HEK293 Cell Line

    E850A2 x 1 vial
2 vials
- E8500 Please Enquire

GloResponse™ NFAT-RE-luc2P HEK293 Cell Line

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GloResponse™ NFAT-RE-luc2P HEK293 Cell Line

 Components

  • GloResponse™ NFAT-RE-luc2P HEK293 Cell Line

    E851A2 x 1 vial
2 vials
- E8510 Please Enquire

GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line

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GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line

 Components

  • NF-κB-luc2P HEK293 Cell Line

    E852A2 x 1 vial
2 vials
- E8520 Please Enquire

GloResponse™ 9XGAL4UAS-luc2P HEK293 Cell Line

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GloResponse™ 9XGAL4UAS-luc2P HEK293 Cell Line

 Components

  • 9XGAL4UAS-luc2P HEK293 Cell Line

    E853A2 x 1 vial
2 vials
- E8530 Please Enquire


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Storage Conditions

Place frozen cells in storage at less than or equal to –140°C (mechanical deep freeze or vapor phase liquid nitrogen) until you are ready to thaw and propagate them. We strongly recommend that the cells are propagated, using the provided procedure, as soon as possible. This will ensure the optimal cell viability and assay performance.

For product intended use please see Patents & Disclaimers tab.

GloResponse CRE-luc2P HEK293 cells response to forskolin titration.Figure 1. GloResponse™ CRE-luc2P HEK293 cells response to forskolin titration.

A total of 10,000 GloResponse™ CRE-luc2P HEK293 cells per well were dispensed into each well of a 96-well plate, and twofold serial dilutions of forskolin were added to induce reporter gene expression. After 4 hours of induction in a tissue culture incubator, luciferase activity was measured using the Dual-Glo® Luciferase Assay System Reagent on the GloMax® 96 Microplate Luminometer (Cat.# E6501; n = 4).

GloResponse NFAT-RE-luc2P HEK293 cells response to PMA titration.Figure 2. GloResponse™ NFAT-RE-luc2P HEK293 cells response to PMA titration.

A total of 10,000 GloResponse™ NFAT-RE-luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and threefold serial dilutions of PMA were added to induce reporter gene expression. After 16 hours of induction in a tissue culture incubator, luciferase activity was quantified using the Dual-Glo® Luciferase Assay System Reagent on the Berthold® LB 96 V Luminometer. n = 8 for each data point.

GloResponse NF-KB-RE-luc2P HEK293 cells response to TNF-alpha titration.Figure 3. GloResponse™ NF-kappa-B-RE-luc2P HEK293 cells response to TNF-alpha titration.

A total of 10,000 GloResponse™ NF-kappa-B-RE-luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and twofold serial dilutions of TNF-alpha were added to induce reporter gene expression. After 5 hours of induction in a tissue culture incubator, luciferase activity was quantified using the ONE-Glo™ Luciferase Assay System Reagent on the Berthold® LB 96 V Luminometer. n = 8 for each data point.

E2 titration of GloResponse 9XGAL4UAS-luc2P HEK293 cells transfected with pBIND-ER-alpha Vector.Figure 4. E2 titration of GloResponse™ 9XGAL4UAS-luc2P HEK293 cells transfected with pBIND-ER-alpha Vector.

A total of 10,000 transfected GloResponse™ 9XGAL4UAS-luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and fourfold serial dilutions of E2 were added to induce reporter gene expression. After a 24-hour induction in a tissue culture incubator, luciferase activity was quantified using the Dual-Glo® Luciferase Assay System Reagent on the Thermo VarioSkan Luminometer. n = 4 for each data point.

Dexamethasone titration of GloResponse 9XGAL4UAS-luc2P HEK293 cells transfected with pBIND-GR Vector.Figure 5. Dexamethasone titration of GloResponse™ 9XGAL4UAS-luc2P HEK293 cells transfected with pBIND-GR Vector.

A total of 10,000 GloResponse™ 9XGAL4UAS-luc2P HEK293 cells were dispensed into each well of a 384-well plate, and fivefold serial dilutions of dexamethasone were added to induce reporter gene expression. After a 24-hour induction in a tissue culture incubator, luciferase activity was quantified using the Dual-Glo® Luciferase Assay System Reagent on the Thermo VarioSkan Luminometer. n = 4 for each data point.

Representation of the one-hybrid system.Figure 6. Representation of the one-hybrid system.

In this system, the nuclear receptor ligand binding domain (LBD) is fused to the DNA-Binding Domain of the yeast GAL4 transcription factor. The hybrid fusion-protein nuclear receptor is then used to activate the luc2P luciferase reporter under the control of nine repeats of the GAL4 Upstream Activator Sequence (UAS).

In this system, the nuclear receptor ligand binding domain (LBD) is fused to the DNA-Binding Domain of the yeast GAL4 transcription factor. The hybrid fusion-protein nuclear receptor is then used to activate the luc2P luciferase reporter under the control of nine repeats of the GAL4 Upstream Activator Sequence (UAS).

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Use Restrictions

E8500, E8510, E8520, E8530 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

E8500, E8510, E8520, E8530 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

E8500, E8510, E8520, E8530 U.S. Pat. No. 5,670,356.

E8500, E8510, E8520, E8530 Patent Pending.

E8500, E8510, E8520, E8530 U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

E8500, E8510, E8520, E8530 Commercial use of this cell line requires a license from AdVec Inc.

E8500, E8510, E8520, E8530 Use of Genetically Modified Microorganisms (GMM)
Information for European Customers: These products are genetically modified as described in Promega technical literature. As a condition of sale, use of this product must be in accordance with all applicable local guidelines on the contained use of genetically modified microorganisms, including the Directive 2009/41/EC of the European Parliament and of the Council.

E8500, E8510, E8520, E8530 HEK293 cells were obtained under license from AdVec Inc.

E8500, E8510, E8520, E8530 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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