Chroma-Glo™ Luciferase Assay System

The Chroma-Glo™ Luciferase Assay System and the Chroma-Luc™ Vectors provide a method to generate red and green (dual-color) luminescence from a single sample upon a single-reagent addition. Filtered measurement of the dual-color luminescence produced by the Chroma-Luc™ luciferases permits each reporter to be measured independently and virtually simultaneously. The Chroma-Glo™ Assay is in a homogeneous format that generates luminescence with >30-minute signal half-lives for each of the Chroma-Luc™ luciferases, thereby enabling the processing of many plates without prior sample handling. Use the high-homology Chr...

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The Chroma-Glo™ Luciferase Assay System and the Chroma-Luc™ Vectors provide a method to generate red and green (dual-color) luminescence from a single sample upon a single-reagent addition. Filtered measurement of the dual-color luminescence produced by the Chroma-Luc™ luciferases permits each reporter to be measured independently and virtually simultaneously. The Chroma-Glo™ Assay is in a homogeneous format that generates luminescence with >30-minute signal half-lives for each of the Chroma-Luc™ luciferases, thereby enabling the processing of many plates without prior sample handling. Use the high-homology Chroma-Luc™ luciferases to establish an ideal internal control for normalizing cytotoxicity in downregulation applications and for decreasing inter- and intrasample variability. You can also use the reporters to multiplex experimental reporters to increase the data content from cell-based assays.

Features - Benefits

• Measure Dual Reporters Using a Single Substrate Addition: Increase your accuracy and precision through normalization, or use both reporters to multiplex experimental measurements. Use filters to spectrally separate the luminescent signals.
• Establish the Ideal Control or Multiplexed System: Use the high-homology red and green luciferases to minimize potential RNA and protein effects on reporter expression.
• Increase Your Throughput: Use the stable luminescence for batch or continuous processing of multiple plates.
• Perform Fewer Steps: Add Chroma-Luc™ Reagent directly to cells in medium, then measure.
• Automate This Assay: Validated automated methods available at: www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

For more information, see the Protocols & Applications Guide.

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