Promega Corporation

MagneHis™ Protein Purification System

The MagneHis™ Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format, polyhistidine- or HQ-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture. Samples can be processed in a high-thro...

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MagneHis™ Protein Purification System

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MagneHis™ Protein Purification System

 Components

  • MagneHis™ Binding/Wash Buffer

    V851A1 x 50ml
  • MagneHis™ Elution Buffer

    V852A1 x 10ml
  • MagneHis™ Ni-Particles

    V854A1 x 2ml
  • FastBreak™ Cell Lysis Reagent, 10X

    V857A1 x 15ml
  • DNase I (lyophilized)

    Z358B1 x 1 vial
65 reactions
- V8500 Please Enquire

MagneHis™ Protein Purification System

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MagneHis™ Protein Purification System

 Components

  • MagneHis™ Binding/Wash Buffer

    V851B1 x 250ml
  • MagneHis™ Elution Buffer

    V852B1 x 50ml
  • MagneHis™ Ni-Particles

    V854B1 x 10ml
  • FastBreak™ Cell Lysis Reagent, 10X

    V857B1 x 60ml
  • DNase I (lyophilized)

    Z358A2 x 1 vial
325 reactions
- V8550 Please Enquire

MagneHis™ Ni-Particles

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MagneHis™ Ni-Particles

 Components

  • MagneHis™ Ni-Particles

    V854A1 x 2ml
2ml
- V8560 Please Enquire

MagneHis™ Ni-Particles

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MagneHis™ Ni-Particles

 Components

  • MagneHis™ Ni-Particles

    V854B1 x 10ml
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  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


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10ml
- V8565 Please Enquire


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Storage Conditions

Store at 4°C.

For product intended use please see Patents & Disclaimers tab.

Figures

Purification of His-tagged methionyl tRNA synthetase.Figure 1. Purification of His-tagged methionyl tRNA synthetase.

Use Restrictions

V8500, V8550, V8560, V8565 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

Cat.# V8500, V8550, V8560, V8565: U.S. Pat. Nos. 7,112,552 and 7,354,750.

Cat.# V8500, V8550: U.S. Pat. No. 7,319,021 and other patents pending.

Cat.# V8500, V8550: Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Cat.# V8500, V8550: This product is licensed for use under U.S. Pat. No. 6,174,704.

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