Promega Corporation

Transcend™ Non-Radioactive Translation Detection Systems

The Transcend™ Non-Radioactive Translation Detection Systems allow non-radioactive detection of proteins synthesized in vitro. Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S]methionine or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosph...

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  • Prices valid for customers of Promega Singapore Pte Ltd only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Transcend™ Colorimetric Translation Detection System

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Transcend™ Colorimetric Translation Detection System

 Components

  • Transcend™ tRNA

    L506A1 x 30μl
  • Western Blue® Stabilized Substrate

    S384C1 x 35ml
  • Streptavidin Alkaline Phosphatase

    V559B1 x 36μl
30 reactions
- L5070 Please Enquire

Transcend™ Chemiluminescent Translation Detection System

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Transcend™ Chemiluminescent Translation Detection System

 Components

  • Transcend™ Chemiluminescent Substrate A

    L203A1 x 25ml
  • Transcend™ Chemiluminescent Substrate B

    L204A1 x 25ml
  • Streptavidin HRP

    L205A1 x 15μl
  • Transcend™ tRNA

    L506A1 x 30μl
30 reactions
- L5080 Please Enquire


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

Transcend™ tRNA

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Transcend™ tRNA

 Components

  • Transcend™ tRNA

    L506A1 x 30μl
30μl
- L5061 Please Enquire

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Storage Conditions

Store Transcend™ tRNA at –70°C. Do not subject the Transcend™ tRNA to more than five freeze-thaw cycles. Store all other components at 4°C.

For product intended use please see Patents & Disclaimers tab.

Structure of Transcend™ tRNA.Figure 1. Structure of Transcend™ tRNA.

Use Restrictions

L5070, L5080, L5061 For Research Use Only. Not for Use in Diagnostic Procedures.

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