Promega Corporation

Immobilized Trypsin

Immobilized Trypsin provides a fast and convenient method for digesting a range of concentrations of purified protein or complex protein mixtures. Digested peptides are easily separated from the Immobilized Trypsin as they flow through the spin column into the collection tube. Immobilized Trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit. Trypsin is a proteolytic enzyme, which cleaves at the carboxyl side of positively charged Lysine (Lys) and Arginine (Arg). When these amino acids are followed by the nonpolar Proline (Pro), the digestion of the site is not ef...

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Immobilized Trypsin

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Immobilized Trypsin

 Components

  • Spin Columns

    V138A2 x 5 each
  • Immobilized Trypsin

    V901B1 x 2ml
2ml
- V9012 Please Enquire

Immobilized Trypsin

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Immobilized Trypsin

 Components

  • Spin Columns

    V138A4 x 5 each
  • Immobilized Trypsin

    V901B2 x 2ml
4ml
(2 × 2ml)
- V9013 Please Enquire


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Storage Conditions

Store at 4°C.

For product intended use please see Patents & Disclaimers tab.

Overview of the Immobilized Trypsin digestion protocol.Figure 1. Overview of the Immobilized Trypsin digestion protocol.

Proteins are denatured, reduced and alkylated as appropriate before digestion. The protein digestion solution, containing the protein to be digested, is prepared and added to the Immobilized Trypsin resin. The protein digestion solution is incubated with the resin for 30 minutes. Digested peptides are removed by centrifugation and then prepared for downstream analysis.

Use Restrictions

V9012, V9013 For Research Use Only. Not for Use in Diagnostic Procedures.

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