simplyRNA kits for the Maxwell® 16 Instrument

Cost Savings

• Complete, all-in-one kits provide all you need
• All reagents are included in the kit, including DNase
• Save by not repeating purifications and failed experiments
• No additional costs for instrument methods or software
• Experience lower total cost of ownership compared to QIAcube® instrument

Comparison of plastic kit consumables used to
process six (6) RNA sample.

Less Space

• Compact solution for automating your sample extractions (less benchtop space)
• Less plastic consumables per kit minimizes storage space

Less Waste

• Fewer plastic consumables in kit compared to other automated commercial kits
• Minimize plastic waste for disposal by using less plastic labware per process

simplyRNA Protocol Overview

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Simplified RNA Extraction

• Reduce pre-extraction handling to 4 steps
• Extract RNA from 16 samples in <1 hour using paramagnetic-particle processing on the Maxwell® 16 instrument
• Avoid complex and time consuming instrument setup associated with the QIAcube® instrument
• No centrifugation required
• No beta-mercaptoethanol (BME) required for sample preparation
• No need to purchase extra items-all consumables included in the kit
• Color coded reagent for DNase addition make it easy to find the correct well

Reliable Performance

• Minimize variability from person to person with automation
• Achieve improved RNA purity and consistent
• Purify RNA for RT-PCR from low cell number and difficult tissues
• Avoid repeat purifications and failed experiments
• Keep RNA intact and contamination-free with minimal sample handling
• Extract RNA from fresh or frozen cells
• Automated particle processing eliminates liquid handling instrument clogging and drips

Intact RNA Extracted from Tissue Using the Maxwell® 16 LEV simplyRNA Tissue Kit

Figure 1. Extracted tissue samples were run on a FlashGel® System for 5 minutes and signal developed for 15 minutes. The 28S, 18S and 5S are clearly visible indicating intact RNA.

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Figure 1. Extracted tissue samples were run on a FlashGel® System for 5 minutes and signal developed for 15 minutes. The 28S, 18S and 5S are clearly visible indicating intact RNA.

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Simplified RNA Extraction

• Minimize sample handling before extraction
• Extract RNA from 16 samples in about an hour using paramagnetic-particle processing on the Maxwell® 16 Instrument
• Lower cost per preparation with no expensive collection tubes required
• Fewer centrifuge steps than other systems
• Use fresh whole blood in either EDTA or PaxGene tubes
• Color coded reagents for DNase addition make it easy to find the correct well

Intact RNA with 28S, 18S and 5S rRNA bands visible

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Reliable Performance

• Achieve improved yield and purity
• Minimize DNA contamination
• Obtain highly concentrated RNA from 2.5ml of whole blood
• Purified RNA ready for down stream applications such as RT-PCR
• Minimize variability from person to person with automation
• Avoid repeat purifications and failed experiments
• Automated particle processing eliminate clogging and drips of the liquid handling instrument
• Obtain purified, intact RNA