ApoLive-Glo™ Multiplex Assay

Predictive Mechanism of Toxicity Determination

• Measure viability and apoptosis in the same sample well: Accurately determine mechanism of cell death in the same sample well, save time and use less cells.
• Easily implement: The assay follows a simple sequential "add-mix-measure" protocol.
• Normalize caspase data with a viability control: The ratio of caspase activity to viable cells is useful for determining the extent of caspase activation and for normalizing cell numbers.
• Flexible and easily automated: Component volumes can be scaled to meet throughput needs, and reagents are robust for automation in 96- to 1536-well plate formats.

Predictive Mechanism of Toxicity Determination

Predictive measures of viability and apoptosis in the same well

Primary Necrosis

• Ionomycin treatment of Jurkat cells for 6 hours
• Dose-dependent decrease in viability
• No caspase-3/7 activation

Apoptosis

• Staurosporine treatment of Jurkat cells for 6 hours
• Dose-dependent decrease in viability
• Increase in caspase-3/7 activation

Simple add-mix-measure protocol and scalable assay reagents make the assay easy to implement and adaptable for 96- to 1536-well formats.

24-hour paclitaxel treatment of Jurkat cells results in dose-dependent decrease in cell viability and increase in caspase-3/7 activity.