Promega Corporation

Pfu DNA PolymerasePCR Satisfaction Guaranteed

Pfu DNA Polymerase is a thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated ...

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  • Prices valid for customers of Promega Singapore Pte Ltd only
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Pfu DNA Polymerase

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Pfu DNA Polymerase

 Components

  • Pfu DNA Polymerase

    M774A1 x 100u
  • Pfu 10X Reaction Buffer w/20mM MgSO4

    M776A1 x 1.2ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100u
2–3u/μl M7741 Please Enquire

Pfu DNA Polymerase

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Pfu DNA Polymerase

 Components

  • Pfu DNA Polymerase

    M774B1 x 500u
  • Pfu 10X Reaction Buffer w/20mM MgSO4

    M776A3 x 1.2ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


500u
2–3u/μl M7745 Please Enquire


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Source

Pyrococcus furiosus strain Vc1 DSM3638.

Storage Buffer

50mM Tris-HCl (pH 8.2 at 25°C), 0.1mM EDTA, 1mM DTT, 50% glycerol and 0.05% CHAPS.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

M7741, M7745 For Research Use Only. Not for Use in Diagnostic Procedures.

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