Promega Corporation

pTargeT™ Mammalian Expression Vector System

The pTargeT™ Mammalian Expression Vector System is a convenient system for cloning PCR products and for expressing cloned PCR products in mammalian cells. The vector is prepared by digestion with EcoRV followed by addition of a 3´ terminal thymidine to each end. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid in two ways. First, the overhangs prevent recircularization of the vector; second, they provide a compatible overhang for PCR products generated by thermostable polymerases that add a single deoxyadenosine, ...

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pTargeT™ Mammalian Expression Vector System

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pTargeT™ Mammalian Expression Vector System

 Components

  • pTargeT™ Vector

    A140A1 x 1.2μg
  • Control Insert DNA

    A363A1 x 12μl
  • T4 DNA Ligase 10X Buffer

    C126B1 x 200μl
  • JM109 Competent Cells, High Efficiency

    L200A6 x 200μl
  • T4 DNA Ligase

    M180A1 x 100u
20 reactions
- A1410 Please Enquire


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GenBank®/EMBL Accession Number

pTargeT™ Mammalian Expression Vector Accession Number AY540613; pTargeT™ Mammalian Expression Vector sequence text file.

Storage Conditions

Store competent cells at –70°C; store all other components at –20°C or –70°C.

For product intended use please see Patents & Disclaimers tab.

pTARGET Mammalian Expression Vector.Figure 1. pTargeT™ Mammalian Expression Vector.

Use Restrictions

A1410 For Research Use Only. Not for Use in Diagnostic Procedures.

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