Sensitive quantitation of RNA is important for the success of downstream applications. The QuantiFluor® RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is a very important step that is used in many biological applications, particularly in molecular biology techniques.
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Each QuantiFluor® RNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl.
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QuantiFluor® RNA System
20X TE Buffer (pH 7.5)
QuantiFluor® RNA Dye
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Store at –30°C to –10°C, protected from light.
For product intended use please see Patents & Disclaimers tab.
The standard curves were generated using RNA Standard in a 96-well format and 200µl total volume as described in Section 5 of the Technical Manual. The standard curve RNA amounts are 2ng, 10ng, 20ng, 50ng, 100ng, 200ng and 500ng per well. Fluorescence was measured using the GloMax®-Multi+ Detection System. The fluorescence values shown were blank-subtracted. Under these conditions, the dynamic range for the QuantiFluor® RNA Dye is approximately 2–500ng per well (in 200µl total volume), and the QuantiFluor® RNA Dye limit of detection is approximately 100pg per well.