Promega Corporation

Caspase-Glo® 6 Assay Systems

The Caspase-Glo® 6 Assay is a homogeneous, luminescent assay that measures caspase-6 activity. Caspase-6 is a member of the cysteine aspartic acid-specific protease family and has a key effector role in the cleavage of specific target proteins during apoptosis. The Caspase-Glo® 6 Assay provides a luminogenic substrate, Z-VEID-aminoluciferin, in a buffer optimized for caspase-6 and luciferase activity. The addition of a single Caspase-Glo® 6 Reagent in an add-mix-measure format results in cleavage of the substrate, releasing aminoluciferin, and generation of a glow-type luminescent signal in the presence of...

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Caspase-Glo® 6 Assay

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Caspase-Glo® 6 Assay

 Components

  • Caspase-Glo® 6 Buffer

    G095A1 x 10ml
  • Z-VEID-Glo™ Substrate

    G096A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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10ml
- G0970 Please Enquire

Caspase-Glo® 6 Assay

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Caspase-Glo® 6 Assay

 Components

  • Caspase-Glo® 6 Buffer

    G095B1 x 50ml
  • Z-VEID-Glo™ Substrate

    G096B1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
50ml
- G0971 Please Enquire


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Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Sensitivity of the Caspase-Glo 6 Assay compared to a fluorescent caspase-6 assay.Figure 1. Sensitivity of the Caspase-Glo® 6 Assay compared to a fluorescent caspase-6 assay.

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Caspase-6 cleavage of the luminogenic substrate containing the VEID consensus sequence.Figure 2. Caspase-6 cleavage of the luminogenic substrate containing the VEID consensus sequence.
Z´-factor value analysis of the Caspase-Glo 6 Assay.Figure 3. Z´-factor value analysis of the Caspase-Glo® 6 Assay.

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Determination of IC50 values with the Caspase-Glo 6 Assay.Figure 4. Determination of IC50 values with the Caspase-Glo® 6 Assay.

Use Restrictions

G0970, G0971 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G0970, G0971 U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and other patents and patents pending.

G0970, G0971 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G0970, G0971 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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